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Biosearch Technologies Inc wash buffer b
Wash Buffer B, supplied by Biosearch Technologies Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wash+buffer+b/a+buffer+wash/pmc12968391-111-9-12
Average 86 stars, based on 1 article reviews
wash buffer b - by Bioz Stars, 2026-09
86/100 stars

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Incubation:

Article Title: Neuronal interactions in forebrain organoids lead to protective antiviral responses.
Article Snippet: .. Cells were pelleted and incubated in wash buffer B (Biosearch Technologies, Inc., CatSMF-WB1-20) for 5 minutes at room temperature. ..

Article Title: Activation of Pvt1b isoform contributes to local Pvt1 abundance to repress Myc during stress.
Article Snippet: Cells were incubated overnight at 30°C with the indicated probes diluted 1:50 in Hybridization solution (Stellaris, Biosearch Technologies) prepared with formamide according to manufacturer’s instructions. .. The next day, cells were washed 2 times for 30 min at 30°C in Wash Buffer A, incubated in Wash Buffer B (Stellaris, Biosearch Technologies) for 5 min at RT, and mounted in antifade reagent (Vectashield Mounting medium with DAPI, Vector Laboratories). .. Images were captured using an Axio Imager 2 microscope system (Zeiss) with a PlanApo 63x 1.4 oil DIC objective lens (Zeiss).

Article Title: C9ORF72 poly-PR induces TDP-43 nuclear condensation via NEAT1 and is modulated by HSP70 activity.
Article Snippet: Article C9ORF72 poly-PR induces TDP-43 nuclear condensation via NEAT1 and is modulated by HSP70 activity

Article Title: A Blueprint of Sex-Specific Neuronal Regulation in the C. elegans Nervous System at Single-Cell Resolution
Article Snippet: .. Then, DAPI was discarded, and worms were added with 1 mL of Wash Buffer B (Biosearch Technologies Cat# SMF-WB1-20) and incubated for 5 minutes at room temperature. .. After discarding Wash Buffer B, 30 μL Vectashield® Mounting Medium (Vector Laboratories Cat #H-1000) were added to the worms for imaging.

Article Title: Repeat-rich RNA guides repetitive genomic elements into biomolecular condensates for heterochromatin organization and muscle integrity
Article Snippet: The cells were then incubated with Fluorescein Avidin DCS (5 μg/ml, Vector Laboratories, A-2011) for 30 min at RT. .. Slides were washed twice for 3 min each with wash buffer B (Biosearch technologies, SMF-WB1-20), followed by incubation with Biotinylated Anti-Avidin (5 μg/ml, Vector Laboratories, BA-0300) for 30 min at RT. ..

Fluorescence In Situ Hybridization:

Article Title: C9ORF72 poly-PR induces TDP-43 nuclear condensation via NEAT1 and is modulated by HSP70 activity.
Article Snippet: Article C9ORF72 poly-PR induces TDP-43 nuclear condensation via NEAT1 and is modulated by HSP70 activity

Hybridization:

Article Title: C9ORF72 poly-PR induces TDP-43 nuclear condensation via NEAT1 and is modulated by HSP70 activity.
Article Snippet: Article C9ORF72 poly-PR induces TDP-43 nuclear condensation via NEAT1 and is modulated by HSP70 activity

Staining:

Article Title: C9ORF72 poly-PR induces TDP-43 nuclear condensation via NEAT1 and is modulated by HSP70 activity.
Article Snippet: Article C9ORF72 poly-PR induces TDP-43 nuclear condensation via NEAT1 and is modulated by HSP70 activity

Article Title: Phase-separated NDF−FACT condensates facilitate transcription elongation on chromatin
Article Snippet: Cells were first incubated in wash buffer A, containing 20% Stellaris RNA-FISH wash buffer A (Biosearch Technologies) and 10% deionized formamide (EMD Millipore) in RNase-free water (Life Technologies), for 5 min. Then, 100 μl of a 12.5 μM human ACTB probe (Biosearch Technologies, ISMF-2003-5) in hybridization buffer, consisting of 90% Stellaris RNA-FISH hybridization buffer (Biosearch Technologies) and 10% deionized formamide, was added to the cells and incubated overnight at 37 °C. .. Post-hybridization, cells were washed for 30 min with wash buffer A at 37 °C, followed by a nuclear stain of 5 ng ml −1 4,6-diamidino-2-phenylindole (DAPI) (Bio-Rad) in wash buffer A for 30 min at 37 °C, and a final 5-min wash in wash buffer B (Biosearch Technologies) at room temperature. .. Coverslips were then mounted on glass slides using Vectashield mounting medium (Vector Laboratories), sealed with nail polish and imaged using a Zeiss LSM800 confocal microscope with a 63× oil objective and Airyscan module.



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